A demonstration of Mycobacterium leprae by electron microscopy.
نویسندگان
چکیده
Se obtuvo el material para examen con el microscopio electronico, de un paciente filepino del "Walter Reed Army Hospital," en Washington, D. C., cuyo diagnostico clinico fue el de lepra con lesiones tanto lepromatosos (nodulares) como tuberculoides. El material se manejo esterilmente y las preparaciones se hicieron antes de dos horas despues de haber sido obtenido. Despues de dejarlas secar durante la noche, las preparaciones fueron plateadas con cromio. Las micrografias electronicas demostraron, en lugar de las formas bacilares que se ven ordinariamente, filamentos extraordinariamente largos semejantes mas al estreptotrix que a los bacilos normales. La pared celular aparecia tener una estructura membranosa "va cia." Se considera interesante el que el bacilo de la lepra pueda producir estas formas extranas.
منابع مشابه
Detection of Non-Tuberculosis Mycobacteria Infection due to Mycobacterium leprae and Mycobacterium kansasii in Patients Suspected of Tuberculosis in Isfahan, Iran
Several reports have indicated that infection with Non-Tuberculosis Mycobacteria (NTM) is increasing worldwide.Therefore,monitoring species causing micobacterial infection in any region is of great importance. This study was going to detect, differentiate, and identify pathogenic mycobacteria in primary clinical samples. Eighty samples collected from tuberculosis suspected patients in Isfahan/I...
متن کاملIL-10 treatment of macrophages bolsters intracellular survival of Mycobacterium leprae.
In these studies, metabolically active Mycobacterium leprae were maintained for as long as 8 weeks in monolayer cultures of mouse peritoneal macrophages (MPhi). Supplemental IL-10, but not TGF-beta, bolstered, directly or indirectly, M. leprae metabolism in mouse MPhi. In the cell culture system temperature setting is extremely important and 31 to 33 degrees C incubation temperature was more pe...
متن کاملObservations on the morphology of Mycobacterium leprae by ordinary optics, phase microscopy, and electron microscopy.
Although in recent years Mycobacterium leprae has been examined to a certain extent with the aid of the phase contrast microscope (7) and by electron microscopy (1,3,5,8), the bulk of our knowledge about its morphology rests on observations made with preparations stained by the Ziehl-N eelsen method and examined with classical optics. The interpertations regarding the biological significance of...
متن کاملA comparative study by electron microscopy of the morphology of Mycobacterium leprae and cultivable species of mycobacteria.
The usefulness of the electron microscope in the study of bacteria is well established. It has provided a new technique for studying the internal structure of the cell, not revealed by the present methods of light microscopy. It should yield clues which will lead to a better understanding of the reactions between infectious and bactericidal agents, as well as those between host and parasite in ...
متن کاملTHE DETECTION OF Mycobacterium tuberculosis BY PCR IN SPUTUM SAMPLES FROM LONG-TERMTREATED LEPROSY PATIENTS: ASSOCIATIONS WITH SKIN TEST RESULTS AND IMMUNOTHERAPY WITH Mycobacterium vaccae
As part of a series of investigations at Baba Baghi Leprosarium in lran, 44 long-treated leprosy patients were selected for our study. Samples of early morning sputum were obtained from each patient, examined by microscopy for acid-fast bacilli (AFB), and cultured for tubercle bacilli. These tests were negative, but the polymerase chain reaction (PCR) for an insertion sequence believed to b...
متن کاملA RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS
We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymer...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- International journal of Leprosy
دوره 20 1 شماره
صفحات -
تاریخ انتشار 1952